251500-M

DAF-2

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

• 別名: DAF-2, 4,5-Diaminofluorescein
• 実験式（ヒル表記法）: C₂₀H₁₄N₂O₅
• CAS番号: 205391-01-1
• 分子量: 362.34
• MDL番号: MFCD05865100
• UNSPSCコード: 12171500
• NACRES: NA.25

特性

• 品質水準: 100
• アッセイ: ≥98% (HPLC)
• フォーム: solution
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: pale yellow
• 蛍光検出: λ_ex ~495 nm; λ_em ~515 nm
• 保管温度: −20°C
• SMILES記法: Nc1cc2c(cc1N)C(=O)OC32c4c(cc(cc4)O)Oc5c3ccc(c5)O
• InChI: 1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
• InChI Key: LTYUPYUWXRTNFQ-UHFFFAOYSA-N

詳細

詳細

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

生物化学的／生理学的作用

Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

警告

Toxicity: Irritant (B)

物理的形状

A 5 mM (1 mg in 550 µl) solution in DMSO.

再構成

Following initial thaw, aliquot and freeze (-20°C).

その他情報

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

法的情報

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• 引火点(℃): 87 °C - closed cup - (Dimethylsulfoxide)