264150

Caspase-3 Substrate IV, Fluorogenic

Fluorogenic caspase-3 substrate.

• 別名: Caspase-3 Substrate IV, Fluorogenic, Z-DEVD-AFC
• 実験式（ヒル表記法）: C₃₆H₃₈F₃N₅O₁₄
• CAS番号: 1177935-21-5
• 分子量: 821.71
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 色: white to off-white
• 溶解性: DMSO: 10 mg/mL
• 蛍光検出: λ_ex ~400 nm; λ_em ~505 nm
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.

Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
Fluorogenic caspase-3 substrate.

Product does not compete with ATP.

Reversible: no

警告

Toxicity: Standard Handling (A)

シーケンス

Z-Asp-Glu-Val-Asp-AFC

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.

その他情報

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable