344906

FURA 2/AM in Solution

• 別名: FURA 2/AM in Solution
• 実験式（ヒル表記法）: C₄₄H₄₇N₃O₂₄
• CAS番号: 108964-32-5
• 分子量: 1001.85
• MDL番号: MFCD00036976
• UNSPSCコード: 12171500
• NACRES: NA.47

特性

• アッセイ: ≥95% (HPLC)
• 品質水準: 100
• 形状: liquid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 蛍光検出: λ_ex 335 nm (high); λ_ex 362 nm (low); λ_em 505 nm (high); λ_em 512 nm (low)
• 輸送温度: ambient
• 保管温度: −20°C
• InChI: 1S/C44H47N3O24/c1-25-7-8-32(46(16-38(53)65-20-60-26(2)48)17-39(54)66-21-61-27(3)49)35(13-25)58-11-12-59-42-31-14-36(43-45-15-37(71-43)44(57)69-24-64-30(6)52)70-34(31)10-9-33(42)47(18-40(55)67-22-62-28(4)50)19-41(56)68-23-63-29(5)51/h7-10,13-15H,11-12,16-24H2,1-6H3
• InChI Key: UTBYGIDPBQOHNT-UHFFFAOYSA-N

詳細

詳細

Cell-permeable ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (K_d = 224 nM) that offers 30X greater fluorescence intensity that QUIN 2 (Cat. No. 551826). This probe has been used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm).

生物化学的／生理学的作用

Cell permeable: no

Product does not compete with ATP.

Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Irritant (B)

物理的形状

A 1 mM solution of FURA 2/AM (Cat. No. 344905) in anhydrous DMSO.

再構成

Following initial thaw, aliquot and freeze (-20°C). Store frozen under an inert atmosphere.

その他情報

Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.
Schwartz, Y., et al. 1992. Endocrinology131, 772.
Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.
Tsien, R.Y. 1980. Biochemistry19, 2396.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• 引火点(℃): 87 °C - closed cup - (Dimethylsulfoxide)