475989

MTT

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.

• 別名: MTT, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
• 実験式（ヒル表記法）: C₁₈H₁₆BrN₅S
• CAS番号: 298-93-1
• 分子量: 414.32
• MDL番号: MFCD00011964
• UNSPSCコード: 12171500
• NACRES: NA.25

特性

• 品質水準: 100
• アッセイ: ≥98% (silver nitrate titration)
• フォーム: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 不純物: ≤0.5% Sulfated ash
• 色: yellow
• 溶解性: PBS: 2 mg/mL
• 微量陽イオン: heavy metals: ≤0.001%
• 輸送温度: ambient
• 保管温度: 15-25°C
• SMILES記法: [s]1c(nc(c1C)C)[n]2[n+](nc(n2)c4ccccc4)c3ccccc3.[Br-]
• InChI: 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
• InChI Key: AZKSAVLVSZKNRD-UHFFFAOYSA-M

詳細

詳細

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
Used as a direct indicator of cytotoxicity and apoptosis.

Product does not compete with ATP.

Reversible: no

警告

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

再構成

Following reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.

その他情報

Liu, Y., et al. 1997. J. Neurochem.69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS08,GHS07
• シグナルワード: Warning
• 危険有害性情報: H315,H319,H335,H341
• 注意書き: P202 - P261 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危険有害性の分類: Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable