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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
C219, monoclonal
形状
liquid
含みます
≤0.1% sodium azide as preservative
化学種の反応性
rat, hamster, human, mouse
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
avoid repeated freeze/thaw cycles
アイソタイプ
IgG1
輸送温度
wet ice
保管温度
−20°C
遺伝子情報
human ... ABCB1(5243)
詳細
Purified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
This Anti-P-Glycoprotein Mouse mAb (C219) is validated for use in FC, Frozen Sections, Immunoblotting, ICC, IP, Paraffin Sections for the detection of P-Glycoprotein.
免疫原
Hamster and Human
SDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a multidrug resistant human cell line
アプリケーション
Flow Cytometry (5-10 µg/1 x 10⁶ cells)
Frozen Sections (5-10 µg/ml)
Immunoblotting (1-10 µg/ml, see application references, see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (1-10 µg/ml)
Frozen Sections (5-10 µg/ml)
Immunoblotting (1-10 µg/ml, see application references, see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (1-10 µg/ml)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
物理的形状
In PBS, 1% BSA.
再構成
Following initial thaw, aliquot and freeze (-20°C).
アナリシスノート
Positive Control
Cell lines or frozen tissue, such as liver or colon
Cell lines or frozen tissue, such as liver or colon
その他情報
Also recognizes ~150 kDa sister P-glycoprotein (SPGP). Frozen sections should be fixed in acetone for 10 min at -20°C. Formalin-fixed, paraffin tissue sections should be deparaffinized and rehydrated by conventional means. Boiling samples may reduce visibility of the protein by immunoblotting (see application references). This antibody is also reported to work for immunocytochemistry and immunoprecipitation. A ~200 kDa protein that migrates in the same position as myosin is also seen by immunoblotting. Variables associated with assay conditions will dictate the proper working dilutions.
Beck, W.T., et al. 1996. Cancer Res. 56, 3010.
Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
法的情報
ImmunoblottingLee, G., et al. 2001. J. Pharmacol. Exp. Ther.299, 204.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
11 - Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
517310-500UL:
517310-UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Linlin Su et al.
The international journal of biochemistry & cell biology, 41(12), 2578-2587 (2009-09-02)
Throughout spermatogenesis, leptotene spermatocytes traverse the blood-testis barrier (BTB) to enter the adluminal compartment of the seminiferous epithelium for continued development. At the same time, the integrity of the BTB, which is constituted by co-existing tight junctions (TJ), basal ectoplasmic
Yan Chen et al.
PloS one, 10(10), e0140918-e0140918 (2015-10-21)
The objective of the present study was to investigate the role of high-mobility group box-1 (HMGB1) in the seizure-induced P-glycoprotein (P-gp) overexpression and the underlying mechanism. Kainic acid (KA)-induced mouse seizure model was used for in vivo experiments. Male C57BL/6
Linlin Su et al.
The Journal of endocrinology, 209(3), 337-351 (2011-04-08)
The blood--testis barrier (BTB) creates an immunological barrier that segregates the seminiferous epithelium into the basal and apical compartment. Thus, meiosis I/II and post-meiotic germ cell development take place in a specialized microenvironment in the apical compartment behind the BTB
Chuan Wang et al.
PloS one, 8(7), e68807-e68807 (2013-07-23)
The etiology of congenital heart defect (CHD) is commonly believed to involve the interaction of multiple environmental and genetic factors. This study aimed to explore the joint effects of the ABCB1 gene C3435T polymorphism and maternal periconceptional toxicants exposure on
Amit J Sabnis et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(32), 9015-9020 (2016-07-28)
Cytosolic and organelle-based heat-shock protein (HSP) chaperones ensure proper folding and function of nascent and injured polypeptides to support cell growth. Under conditions of cellular stress, including oncogenic transformation, proteostasis components maintain homeostasis and prevent apoptosis. Although this cancer-relevant function
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