539481-M

PKA, Catalytic Subunit, Mouse, Recombinant, E. coli

• 別名: cAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
• Enzyme Commission number: 2.7.1.37 (BRENDA, IUBMB)
• UNSPSCコード: 12352202

特性

• 形状: liquid
• 品質水準: 100
• 比活性: ≥2.5 x 10⁶ U/mL
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• その他の活性: Phosphatases, proteases, none detected
• 保管温度: −20°C

詳細

詳細

Recombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca²⁺ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.

Recombinant, mouse PKA catalytic subunit expressed in E. coli. The catalytic subunit of protein kinase A that combines with the regulatory subunit, in the absence of cAMP, to form the inactive PKA holoenzyme. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation. PKA-Cα is reported to potentiate IP₃-induced Ca²⁺ mobilization in isolated hepatocytes. Suitable for studies of PKA phosphorylation in vitro with purified substrates or following the introduction into intact cells or cell extracts.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5.

物理的形状

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.

再構成

Following initial thaw, aliquot and freeze (-20°C).

その他情報

Grant, B.D., and Adams, J.A. 1996. Biochemistry35, 2022.
Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem.271, 16553.
Adams, J.A., et al. 1995. Biochemistry 34, 2447.
Bosma, M.M., et al. 1993. J. Neurosci.13, 5242.
Brindle, P., et al. 1993. Nature364, 821.
Hajnoczky, G., et al. 1993. Biochem. J.293, 413.
Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA90, 10135.
Wang, L-Y., et al. 1993. Science259, 1173.
Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem.264, 20940.
Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1300.
Walsh, D.A., et al. 1968. J. Biol. Chem.243, 3763.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable