71136
Rosetta-gami B(DE3) Competent Cells - Novagen
Escherichia coli, rod shaped
別名:
Competent Cells, Novagen Competent Cells, Rosetta-gami Competent Cells
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About This Item
UNSPSCコード:
41106202
NACRES:
NA.81
由来生物:
Escherichia coli
成長モード:
adherent or suspension
形態:
rod shaped
メーカー／製品名:
Novagen®
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製品名
Rosetta-gami B(DE3) Competent Cells - Novagen, Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.
由来生物
Escherichia coli
品質水準
メーカー／製品名
Novagen®
保管条件
OK to freeze
avoid repeated freeze/thaw cycles
成長モード
adherent or suspension
形態
rod shaped
テクニック
microbiological culture: suitable
輸送温度
dry ice
保管温度
−70°C
関連するカテゴリー
詳細
Genotype: F–ompT hsdSB (rB– mB–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pRARE (CamR, KanR, TetR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta-gami B strains combine the key features of BL21 (and its Tuner derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.
構成
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlRosetta-gami B(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•10 µl10 µlTest Plasmid
•2 × 0.2 ml5 × 0.2 mlRosetta-gami B(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•10 µl10 µlTest Plasmid
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
法的情報
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
10 - Combustible liquids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Youxian Li et al.
Nature, 609(7927), 582-589 (2022-09-08)
Increased levels of proteases, such as trypsin, in the distal intestine have been implicated in intestinal pathological conditions1-3. However, the players and mechanisms that underlie protease regulation in the intestinal lumen have remained unclear. Here we show that Paraprevotella strains
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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