AB5320A4

Anti-NG2, Alexa Fluor^™488 Conjugate Antibody

from rabbit, ALEXA FLUOR^™ 488

• 別名: Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 488
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: rat
• 化学種の反応性（ホモロジーによる予測）: monkey (based on 100% sequence homology), human (based on 100% sequence homology), mouse (based on 100% sequence homology)
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable
• NCBIアクセッション番号: NP_001888
• UniProtアクセッション番号: Q6UVK1
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CSPG4(1464) ; mouse ... Cspg4(121021) ; rat ... Cspg4(81651) ; rhesus monkey ... Cspg4(713086)

詳細

詳細

NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.

特異性

This antibody identifies the intact proteoglycan and core protein by WB and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.

免疫原

Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経変性疾患

Anti-NG2, Alexa Fluor^™488 Conjugate Antibody detects level of NG2 & has been published & validated for use in ICC & IHC.

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected NG2 in adult rat brain tissue.

品質

Evaluated by Immunocytochemistry in rat oligodendrocyte precursor cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton.

ターゲットの説明

The unconjugated parent antibody (Cat. No. AB5320) has an observed molecular weight at ~270-300 kDa

物理的形状

Protein A adsorption

Purified rabbit polyclonal conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

保管および安定性

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

アナリシスノート

Control
Rat or Human oligodendrocyte precursor cells

法的情報

ALEXA FLUOR is a trademark of Life Technologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable