おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
mouse, human, rat
メーカー／製品名
Chemicon®
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... RYR2(6262)
特異性
Ryanodine Receptor 2.
免疫原
Synthetic peptide from the variant TM region of human Ryanodine Receptor 2.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
イオンチャネル及びトランスポーター
神経シグナリング
イオンチャネル及びトランスポーター
神経シグナリング
Anti-Ryanodine Receptor 2 Antibody is an antibody against Ryanodine Receptor 2 for use in IC, IH & WB.
Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 2 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.
Optimal working dilutions must be determined by the end user.
物理的形状
Rabbit serum. Liquid.
保管および安定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
アナリシスノート
Control
Western blot = adult mouse cardiac muscle or brain (adult mouse skeletal muscle will be negative).
IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).
Western blot = adult mouse cardiac muscle or brain (adult mouse skeletal muscle will be negative).
IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB9080:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Effects of CaMKII-mediated phosphorylation of ryanodine receptor type 2 on islet calcium handling, insulin secretion, and glucose tolerance.
Dixit, SS; Wang, T; Manzano, EJ; Yoo, S; Lee, J; Chiang, DY; Ryan, N; Respress et al.
Testing null
Veronica Sacchi et al.
Journal of the American Heart Association, 6(10) (2017-10-12)
Aberrant Ca2+ handling is a prominent feature of heart failure. Elucidation of the molecular mechanisms responsible for aberrant Ca2+ handling is essential for the development of strategies to blunt pathological changes in calcium dynamics. The peptidyl-prolyl cis-trans isomerase peptidyl-prolyl isomerase
Localization and phenotype-specific expression of ryanodine calcium release channels in C57BL6 and DBA/2J mouse strains.
Huang, W; Xing, W; Ryskamp, DA; Punzo, C; Krizaj, D
Experimental Eye Research null
Xiang-Qun Hu et al.
Hypertension (Dallas, Tex. : 1979), 77(4), 1412-1427 (2021-03-02)
[Figure: see text].
A calcium-induced calcium release mechanism supports luteinizing hormone-induced testosterone secretion in mouse Leydig cells.
Costa, RR; Varanda, WA; Franci, CR
American Journal of Physiology. Cell Physiology null
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