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由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, mouse, rat
メーカー／製品名
Chemicon®
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... RYR1(6261)
特異性
Ryanodine Receptor 1.
免疫原
Synthetic peptide from the variant TM region of human Ryanodine Receptor 1.
アプリケーション
Anti-Ryanodine Receptor 1 Antibody detects level of Ryanodine Receptor 1 & has been published & validated for use in IC, IH & WB.
Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 1 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.
Optimal working dilutions must be determined by the end user.
アナリシスノート
Control
Western blot = adult mouse skeletal muscle (adult mouse cardiac muscle will be negative).
IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).
Western blot = adult mouse skeletal muscle (adult mouse cardiac muscle will be negative).
IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB9078:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Diurnal and nutritional adjustments of intracellular Ca2+ release channels and Ca2+ ATPases associated with restricted feeding schedules in the rat liver.
Baez-Ruiz, A; Cazares-Gomez, K; Vazquez-Martinez, O; Aguilar-Roblero, R; Diaz-Mu?oz, M
Journal of circadian rhythms null
Wenli Wang et al.
Fundamental research, 3(3), 459-468 (2022-03-09)
Sweet and umami tastes are elicited by sweet and umami receptors on the tongue and palate epithelium, respectively. However, the molecular machinery allowing the taste reaction remains incompletely understood. Through a phosphoproteomic approach, we identified the key proteins that trigger
A calcium-induced calcium release mechanism supports luteinizing hormone-induced testosterone secretion in mouse Leydig cells.
Costa, RR; Varanda, WA; Franci, CR
American Journal of Physiology. Cell Physiology null
Spaceflight regulates ryanodine receptor subtype 1 in portal vein myocytes in the opposite way of hypertension.
Dabertrand, F; Porte, Y; Macrez, N; Morel, JL
Journal of Applied Physiology (1985)
Topaz Altman et al.
Journal of cell science, 132(23) (2019-11-15)
The neuromuscular junction (NMJ) is the largest, most-complex synapse in the human body. Motor neuron (MN) diseases, such as amyotrophic lateral sclerosis (ALS), specifically target MNs and the NMJs. However, little is known about the reasons for MN-selective neuronal and
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