AB9220

Anti-Nanog Antibody

serum, Chemicon^®

• 別名: Anti-NANOG/STM1, Anti-STM1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: FACS; WB
• 化学種の反応性: mouse, human
• テクニック: flow cytometry: suitable; western blot: suitable
• citations: 20

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: mouse, human
• メーカー／製品名: Chemicon^®
• テクニック: flow cytometry: suitable; western blot: suitable
• 入力: sample type: mouse embryonic stem cell(s); sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• NCBIアクセッション番号: NM_024865.2
• UniProtアクセッション番号: Q9H9S0
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NANOG(79923) ; mouse ... Nanog(71950)

詳細

詳細

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).

特異性

Recognizes Nanog, human.

免疫原

Synthetic peptide from human Nanog.

アプリケーション

Research Category
幹細胞研究

Research Sub Category
多能性及び初期分化

This Anti-Nanog Antibody is validated for use in FC, WB for the detection of Nanog.

Western blotting: 1:1,000-1:5,000. The antibody recognizes a protein of ~35 kDa on MBL-5 cell lysate. Additional bands of ~50 and ~60 kDa may be seen depending on sample and antibody dilution used.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.

Optimal working dilutions must be determined by the end user.

ターゲットの説明

39 kDa (MW 34 kDa)

物理的形状

Unpurified

Rabbit Serum. Liquid.

保管および安定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

アナリシスノート

Control
Whole cell lysate from mouse embryonic stem cells

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable