ABN1018

Anti-LRP1 Antibody, Intracellular domain

serum, from rabbit

• 別名: Prolow-density lipoprotein receptor-related protein 1, A2MR, Alpha-2-macroglobulin receptor, APOER, Apolipoprotein E receptor, CD91, LRP-1, LRP-85, LRPICD
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: mouse
• 化学種の反応性（ホモロジーによる予測）: human (based on 100% sequence homology), rat (based on 100% sequence homology)
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_002323
• UniProtアクセッション番号: Q07954
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... LRP1(4035)

詳細

詳細

Prolow-density lipoprotein receptor-related protein 1 (UniProt Q07954; also known as A2MR, Alpha-2-macroglobulin receptor, APOER, Apolipoprotein E receptor, CD91, LRP-1) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID 4035) in human. LRP1 is an LDLR family member expressed by vascular endothelial cells forming the blood-brain barrier (BBB), vascular mural cells (pericytes and vascular smooth muscle cells (VSMCs)), neurons, and astrocytes. LRP1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (apoE), APP and Aβ. LRP1 is the primary receptor mediating transport of Aβ across the BBB into circulation, thereby clearing it from the brain. LRP1 is synthesized with a signal peptide sequence (a.a. 1-19), posttranslational cleavage yields a soluble form (sLRP1) and a cell surface-bound form composed of an 515 kDa extracellular α-chain (LRP-515) and a 85 kDa single-transmembrane (a.a. 4420-4444) β-chain (LRP-85) that contains the 100-amino intracellular domain (LRPICD; a.a. 4445-4544). BACE-1, ADAM-10, ADAM-12, ADAM-17, MT1-MMP, and t-PA are shown to be involved in the shedding of LRP1 to produce sLRP1.

特異性

Recognizes the Pro- and mature LRP-1 (a.a. 1-4544 and 20-4544, respectively), the beta-secretase-cleaved, membrane-bound LRP-85, and the gamma-secretase-cleaved intracellular domain LRPICD fragment, but not the beta-secretase-cleaved extracellular LRP-515 fragment.

免疫原

Epitope: LRPICD.

GST-tagged recombinant human LRP1 intracellular domain (LRPICD).

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経変性疾患

Anti-LRP1 Antibody, Intracellular domain is an antibody against LRP1 for use in Western Blotting.

Western Blotting Analysis: A representative lot detected the LRP-85 target band in NIH/3T3 cell lysate, as well as in mouse brain and liver homogenates (Courtesy of Professor Nicola Zambrano, University of Naples Federico II, Italy).
Western Blotting Analysis: A representative lot detected a decreased LRP-85 target band in lysates from LRP1 shRNA-treated NIH/3T3 cells (Courtesy of Professor Nicola Zambrano, University of Naples Federico II, Italy).

品質

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected the LRP-85 target band in 10 µg of NIH/3T3 cell lysate.ysate.

ターゲットの説明

~80 kDa observed. Uncharacterized band(s) may appear in some lysates.

物理的形状

Unpurified

Rabbit polyclonal antibody serum with 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable