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主要文書

安全性情報

ABS194

Sigma-Aldrich

Anti-phospho PDHE1-A type I (Ser300) Antibody

from rabbit, purified by affinity chromatography

別名:

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rabbit

品質水準

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

primary antibodies

クローン

polyclonal

精製方法

affinity chromatography

化学種の反応性

human

化学種の反応性(ホモロジーによる予測)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology), zebrafish (based on 100% sequence homology)

テクニック

immunocytochemistry: suitable
western blot: suitable

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

wet ice

ターゲットの翻訳後修飾

phosphorylation (pSer300)

遺伝子情報

human ... PDHA1(5160)

詳細

In many organisms, the pyruvate dehydrogenase complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic, energy-generating pathways, in addition to serving as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.

特異性

This antibody recognizes PDHE1-A type I phosphorylated at Ser300.

免疫原

Epitope: Phosphorylated Ser300
KLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser300.

アプリケーション

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PDHE1-A type I in dichloroacetate untreated and treated HEK 293 cells.
This Anti-phospho PDHE1-A type I (Ser300) Antibody is validated for use in WB, IC for the detection of phospho PDHE1-A type I (Ser300).

品質

Evaluated by Western Blot in dichloroacetate untreated and treated HEK 293 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PDHE1-A type I on 10 µg of dichloroacetate untreated and treated HEK 293 cell lysates.

ターゲットの説明

~43 kDa observed

アナリシスノート

Control
Dichloroacetate untreated and treated HEK 293 cell lysates

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

ABS194:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Jinxin Liu et al.
Theranostics, 11(17), 8283-8300 (2021-08-11)
Rationale: The molecular mechanisms underlying the pathogenesis of systemic insulin resistance in type 2 diabetes remain elusive. Growth hormone receptor (GHR) deficiency has long been known to improved insulin sensitivity. However, whether hepatic GHR overexpression or activation is a cause
Yuan Z Feng et al.
Journal of lipid research, 58(11), 2147-2161 (2017-08-22)
Lipid droplet (LD) coating proteins are essential for the formation and stability of intracellular LDs. Plin2 is an abundant LD coating protein in skeletal muscle, but its importance for muscle function is unclear. We show that myotubes established from Plin2-/-
Kenichi Aizawa et al.
Cells, 13(1) (2024-01-11)
Heart failure with reduced ejection fraction (HFrEF) is characterized not only by reduced left ventricular ejection fraction (EF) but is also combined with symptoms such as dyspnea, fatigue, and edema. Several pharmacological interventions have been established. However, a treatment targeting
G Perez-Siles et al.
Scientific reports, 10(1), 9262-9262 (2020-06-07)
Charcot-Marie-Tooth (CMT) is a group of inherited diseases clinically and genetically heterogenous, characterised by length dependent degeneration of axons of the peripheral nervous system. A missense mutation (p.R158H) in the pyruvate dehydrogenase kinase 3 gene (PDK3) has been identified as
Anders Kalsen et al.
Journal of applied physiology (Bethesda, Md. : 1985), 117(10), 1180-1187 (2014-09-27)
In a randomized, double-blind crossover design, we investigated the effect of the beta2-agonist terbutaline (TER) on endurance performance and substrate utilization in nine moderately trained men [maximum oxygen uptake (V̇O(2 max)) 58.9 ± 3.1 ml·min(-1)·kg(-1)]. Subjects performed 60 min of

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