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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
クローン
1C8, monoclonal
化学種の反応性
human
メーカー/製品名
Chemicon®
テクニック
ELISA: suitable
immunofluorescence: suitable
western blot: suitable
アイソタイプ
IgG2b
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
詳細
Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia.
特異性
Reacts with human Erythropoietin (Epo).
免疫原
Recombinant human Epo.
アプリケーション
Research Category
代謝
代謝
Research Sub Category
代謝ホルモン及び受容体
代謝ホルモン及び受容体
Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.
ELISA:
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.
Immunosorbent preparation
Optimal working dilutions must be determined by the end user.
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.
Immunosorbent preparation
Optimal working dilutions must be determined by the end user.
品質
Evaluated by Western Blot on Mouse kidney lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.
ターゲットの説明
34 kDa (glycosylated)
物理的形状
Protein A purified
Format: Purified
Purified mouse monoclonal IgG2b in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
保管および安定性
Stable for 1 year at 2-8ºC from date of receipt.
アナリシスノート
Control
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB1072:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
PloS one, 8(11), e78627-e78627 (2013-11-14)
Recent advancements in isolation techniques for cytochrome c (Cytc) have allowed us to discover post-translational modifications of this protein. We previously identified two distinct tyrosine phosphorylated residues on Cytc in mammalian liver and heart that alter its electron transfer kinetics
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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