MAB19562X

Anti-Laminin-5 Antibody, clone D4B5, Alexa Fluor^™ 488 conjugated

clone D4B5, Chemicon^®, from mouse

• 別名: Anti-B2T, Anti-CSF, Anti-EBR2, Anti-EBR2A, Anti-LAMB2T, Anti-LAMNB2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 488
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: D4B5, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Chemicon^®
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NM_005562.2; NM_018891.2
• UniProtアクセッション番号: Q13753
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... LAMC2(3918)

詳細

詳細

Laminins are basement membrane, extracellular matrix glycoproteins that are composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes. Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease. LN5 may stabilize the epidermal/dermal junction of skin through binding with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. Compared to fibronectin, collagen, or vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent. Recently, Laminin-5 is attracting attention as a substrate for carcinoma cells that may stimulate migration, since it has been detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, et al., 2002, Lohi, et al., 2001).

特異性

Monoclonal antibody MAB19562 was developed against amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein. The antibody recognizes the 150 kDa (precursor form) and 105 kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.

免疫原

Amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein.

Epitope: amino acids 382-608

アプリケーション

Research Category
細胞骨格

Research Sub Category
ECMタンパク質

Immunocytochemistry: 2 μg/mL
Flow cytometry
Optimal working dilutions must be determined by the end user.

This Anti-Laminin-5 Antibody, clone D4B5, Alexa Fluor 488 conjugated is validated for use in FC, IC for the detection of Laminin-5.

物理的形状

Purified immunoglobulin conjugated to Alexa Fluor^™ 488. Liquid in PBS containing 1% BSA, 0.05% Tween-20 and 0.05% sodium azide.

保管および安定性

Maintain at 2°-8°C for up to 12 months from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Alexa Fluor^™ is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable