MAB3369

Anti-MMP-3 Antibody, clone SL-1 IID4

clone SL-1 IID4, Chemicon^®, from mouse

• 別名: Stromelysin-1, Transin, Proteoglycanase
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: SL-1 IID4, monoclonal
• application: ELISA; IHC; IP; WB
• 化学種の反応性: human
• テクニック: ELISA: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• citations: 5

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: SL-1 IID4, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2b
• NCBIアクセッション番号: NM_002422.3
• UniProtアクセッション番号: P08254
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MMP3(4314)

詳細

詳細

MMPs are frequently expressed in malignant neoplastic cells and play crucial roles in tumor invasion and metastasis. Tissue inhibitors of matrix metalloproteinases (TIMPs) control the activitiy of MMPS. The tissue distribution of three major MMPs has been defined in human mammary pathology: 1) collagenase (MMP-1) which degrades fibrillar interstitial collagens; 2) a 72-k/Da gelatinase (MMP-2) which mainly degrades type IV collagen and denatured collagens; and 3) stromelysin (MMP-3) which has a wider range of action, degrading several matrix components including the core proteins of proteoglycans, laminin and non-helical regions of collagens.

特異性

MAB3369 recognizes one doublet of 54kDa/59kDa and another doublet of 44kDa/49kDa, which are identified as unglycosylated and glycosylated species of pro (latent) and active forms of matrix metalloproteinase-3 (MMP-3; also known as Stromelysin-1 of Transin). MAB3369 shows no cross-reaction with the pro and active forms of other MMPs.

CELLULAR LOCALIZATION:

Cytoplasmic

免疫原

APMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1

アプリケーション

Research Category
細胞骨格

Research Sub Category
MMP及び組織メタロプロテアーゼ

Anti-MMP-3 Antibody, clone SL-1 IID4 is an antibody against MMP-3 for use in ELISA, IP, WB, IH(P).

Immunofluorescence

Immunoprecipitation (Use Protein A): 2μg/ml protein lysate.

Western blotting: 0.5-1.0 μg/ml for 2hrs at RT

Immunohistology (frozen & formalin-fixed): 10-20 μg/ml for 60 minute at room temperature. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissue sections.

Optimal working dilutions and protocols must be determined by end user.

物理的形状

Format: Purified

Purified from ascites fluid by Protein A chromatography. 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

保管および安定性

Antibody with sodium azide is stable for 12 months when stored at 2-8°C.

アナリシスノート

Control
POSTIVE CONTROL:

Human endometrium cells, placenta, bladder, breast, ovarian carcinomas.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable