MAB3828

Anti-Mucin 5B Antibody, clone 15.5B

clone 15.5B, Chemicon^®, from mouse

• 別名: MUC5B
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43
• クローン: 15.5B, monoclonal
• application: ELISA; IHC; WB
• 化学種の反応性: human, monkey
• テクニック: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• citations: 6

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 15.5B, monoclonal
• 化学種の反応性: human, monkey
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG2b
• NCBIアクセッション番号: NM_002458.1
• UniProtアクセッション番号: Q9HC84
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MUC5B(727897)

詳細

詳細

Mucins are a family of highly glycosylated proteins that are present on the surface of, and are secreted by, many glandular epithelial cells. Mucins provide a barrier between the luminal membranes of cells in the epithelium and their environment. For instance, in the digestive tract, the mucin barrier protects the epithelial cells from the action of proteolytic enzymes. These high molecular weight glycoproteins consist of a nonglobular protein backbone (apomucin) and many O-linked glycans. Salivary Mucin 5B proteins are some of the largest polypeptides, with molecular weights ranging from 14 - 40 x 10³ kDa.

特異性

Reacts with Mucin 5B (MUC5B). Does not react with human Mucin 5A or 5C.

免疫原

Un-glycosylated, serine- and threonine-free synthetic peptide from the central region of human MUC5B.

アプリケーション

Research Category
アポトーシス及び癌

Research Sub Category
腫瘍マーカー

This Anti-Mucin 5B Antibody, clone 15.5B is validated for use in ELISA, WB, IH for the detection of Mucin 5B.

Western blot: Bands in the high molecular weight range of 200kDa or above are expected for highly glycosylated proteins.

Immunohistchemistry: Paraffin sections. Deglycosylating the tissue is critical to expose epitope. There are a number of deglycosylation protocols available and a discussion of these related to Mucin can be found in Cao, Y. et al. (1997)(see references).

ELISA

Optimal working dilutions must be determined by end user.

物理的形状

Format: Purified

Purified immunoglobulin. Liquid in PBS containing 0.1% sodium azide.

保管および安定性

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable