MABS341

Anti-LRP6 Antibody, clone 1C10

clone 1C10, from mouse

• 別名: Low-density lipoprotein receptor-related protein 6, LRP-6
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 1C10, monoclonal
• 化学種の反応性: human, rat, mouse
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_002327
• UniProtアクセッション番号: O75581
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... LRP6(4040)

詳細

詳細

Low density lipoprotein receptor-related protein 6, also known as LRP6, is a member of the low density Lipoprotein receptor gene family (LDLR). LDLR are cell surface proteins. LRP6 plays a crucial role in embryonic development by functioning as a co-receptor for the Wnt signaling pathway. LRP6 is highly homologous and presents itself as a dimer in the cell membrane until it is phosphlorylated by Wnt. LRP6 has a high affinity with the Dickkopf (DKK) family of antagonists which, when bound, block Wnt. When LRP6 binds to Wnt it is phosphlorated at multiple sites, which results in Axin, a scaffolding protein, being recruited to the cell membrane and β-catenin activation. β-catenin prevents Wnt and LRP6 from binding. LRP6 is associated with autosomal dominant coronary artery disease type 2. Mutations resulting in the omission of the extracellular domain of LPR6 results in constant activity whereas carboxy intracellular domain exclusion has a negative effect, resulting in the blocking of Wnt signaling.

免疫原

Flag-tagged recombinant protein corresponding to human LRP6.

アプリケーション

Immunoprecipitation Analysis: A representative lot immunoprecipitated LRP6 in certain cell lyates (Khan, Z., et al. (2007). Mol Cell Biol. 27(20):7291-7301.).

This LRP6 antibody is validated for use in WB & IP for the detection of the LRP6 protein.

品質

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected LRP6 in 10 µg of HEK293 cell lysate. For enhanced results, it is recommended for end-users to perform higher dilutions of this antibody.

ターゲットの説明

~200 kDa observed

物理的形状

Format: Purified

Purified mouse monoclonal IgG containing PBS with 0.05% sodium azide.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable