10378445001

Chromozym PK

90% (enzymatic), powder, pkg of 20 mg

• 別名: C33H39N8O6COOCH3
• UNSPSCコード: 12352204

特性

• 詳細: Molecular Formula: C₃₃H₃₉N₈O₆COOCH₃
• 品質水準: 100
• アッセイ: 90% (enzymatic)
• フォーム: powder
• 分子量: M_r 702.9
• 包装: pkg of 20 mg
• メーカー／製品名: Roche
• 保管温度: room temp

詳細

詳細

Chromozym^® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.

アプリケーション

Use Chromozym^® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.

品質

Contaminant: <0.5% free 4-nitraniline

シーケンス

Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate

原理

Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.

調製ノート

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.

その他情報

For life science research only. Not for use in diagnostic procedures.

法的情報

Chromozym is a registered trademark of Pentapharm AG, Basel

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable