T15760

トリズマ^® 塩酸塩 塩酸塩

reagent grade, ≥99%, crystalline

• 別名: TRIS HCl, TRIS 塩酸塩, トリス(ヒドロキシメチル)アミノメタン 塩酸塩, トロメタン塩酸塩
• 化学式: NH₂C(CH₂OH)₃ · HCl
• CAS番号: 1185-53-1
• 分子量: 157.60
• Beilstein: 3675235
• MDL番号: MFCD00012590
• UNSPSCコード: 12161700
• NACRES: NA.25

特性

• 由来生物: synthetic
• 品質水準: 200
• グレード: reagent grade
• アッセイ: ≥99%
• フォーム: crystalline
• 保管条件: (Tightly closed. Dry)
• 不純物: <0.5% water
• 色: white
• 有効pH範囲: 7.0-9.0
• pKa (25 °C): 8.1
• mp: ~150.7 °C
• 溶解性: water: 1.5 g/mL, clear, colorless
• 微量陽イオン: Fe: ≤5 ppm; Pb: ≤5 ppm
• 保管温度: room temp
• SMILES記法: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

詳細

詳細

Tris HCl, or Trizma^® hydrochloride, serves as a widely utilized acidic buffer with a pKa of 8.08 at 25°C, offering a pH range of 7.0 to 9.0, aligning with the typical pH of living organisms. It is a crucial component in buffer solutions for diverse biological, cell biology, and biochemistry applications. Notably, Tris base and Tris hydrochloride individually lack sufficient buffering capacity, necessitating their combination to create effective buffers within the pH range of 7 to 9.

Essential in protein electrophoresis and western blotting techniques, Tris buffers, particularly low ionic strength versions, are employed for protein transfer. They are also commonly used in SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, integral to DNA agarose electrophoresis, contribute to buffer preparations like TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA), playing pivotal roles in biochemistry, protein purification, and electrophoresis applications. Overall, Tris HCl proves to be a versatile and indispensable component, ensuring optimal pH in biological systems for effective biochemical and cell biology research.

特徴および利点

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

その他情報

すべてのバッファーのpHは、温度や濃度に依存します。トリスバッファーの場合、温度が1°C低下するとpHは約0.03上昇し、希釈10倍ごとにpHは0.03～0.05低下します。精密なアプリケーションには、適切に校正した、ガラス／カロメル複合電極のpHメーターを使用してください。

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The pH values of all buffers are temperature and concentration-dependent. For Tris buffers, pH increases by about 0.03 unit per °C decrease in temperature and decreases by 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

法的情報

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable