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由来生物
goat
品質水準
結合体
alkaline phosphatase conjugate
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
secondary antibodies
クローン
polyclonal
形状
buffered aqueous solution
化学種の反応性
mouse
以下との反応禁止
human, rat
テクニック
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10
western blot: 1:200,000-1:400.000 using assay detecting β-Actin in total cell extract of HeLa cells (5-10 μg per well)
輸送温度
wet ice
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
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詳細
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody detects mouse IgG (whole molecule). When tested by ELISA, the product reacts with the Fab fragment of mouse IgG but does not recognize the Fc fragment of mouse IgG, human IgG, IgA, IgM or rat IgG.
免疫原
Purified mouse IgG
アプリケーション
Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.
Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is suitable for use in western blot at 1:1000 dilution using human airway epithelial cells or using whole-cell extracts of rat tubal tissues . The antibody can also be used for immunohistochemistry (1:10 using formalin-fixed, paraffin-embedded sections).
Goat anti-mouse Fab specific alkaline phosphatase antibody was used to analyze serum Ig titers by His Sorb ELISA at 1:10000 at room temperature for two hours. Antibodies were diluted in PBS plus 0.2% BSA to reduce background.
その他情報
Antibody adsorbed with human IgG and rat serum proteins.
物理的形状
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 with 50% glycerol as stabilizer and 15 mM sodium azide
調製ノート
バックグラウンド染色を低減するため、ヒトまたはラットサンプルで吸収済みです。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A1682-.5ML:
A1682-VAR:
A1682-.25ML:
A1682-BULK:
A1682-1ML:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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