A7340
抗グルタチオン-S-トランスフェラーゼ (GST)–ペルオキシダーゼ標識 ウサギ宿主抗体
IgG fraction of antiserum, buffered aqueous solution
別名:
抗グルタチオン-S-トランスフェラーゼ, 抗GST抗体
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About This Item
UNSPSCコード:
12352203
NACRES:
NA.46
結合体:
peroxidase conjugate
application:
ELISA (d)
WB
WB
クローン:
polyclonal
テクニック:
direct ELISA: 1:10,000
western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST
western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST
citations:
35
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由来生物
rabbit
品質水準
結合体
peroxidase conjugate
抗体製品の状態
IgG fraction of antiserum
抗体製品タイプ
primary antibodies
クローン
polyclonal
フォーム
buffered aqueous solution
分子量
antigen 27.5 kDa
テクニック
direct ELISA: 1:10,000
western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
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詳細
本抗体は、Schistosoma japonicum（日本住血吸虫）由来のグルタチオン-S-トランスフェラーゼの未変性型ならびに変性・還元型に特異的です。抗GSTは、GST融合タンパク質の発現を確認するために、様々な免疫アッセイに使用される場合があります。
Glutathione-S-Transferase (GST) belongs to a family of Phase II detoxification enzymes which are involved in catalyzing the conjugation of glutathione (GSH) with many exogenous and endogenous electrophilic substances. GST is of two kinds- membrane-bound microsomal and cytosolic. The cytosolic GSTs are made of six classes: α, μ, ω, π, θ, and ζ.
特異性
Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.
免疫原
大腸菌で発現させた日本住血吸虫由来のリコンビナントGSTです。
アプリケーション
Anti-Glutathione-S-Transferase (GST)-Peroxidase Conjugate antibody has been used in
- immunoblotting
- affinity pull-down assay
- western blotting
- enzyme linked immunosorbent assay (ELISA)
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
生物化学的／生理学的作用
Glutathione-S-Transferase (GST) are involved in protecting cellular macromolecules from the reactive electrophiles. The μ and π transferases regulate the mitogen-activated protein (MAP) kinase pathway. GSTs help in building up cellular resistance against microbial antibiotic, herbicides, insecticides, chemotherapy agents and drugs.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.
調製ノート
Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978)による2段階グルタルアルデヒド法で調製しています
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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シグナルワード
Danger
危険有害性情報
危険有害性の分類
Resp. Sens. 1 - Skin Sens. 1
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A7340-VAR:
A7340-BULK:
A7340-.5ML:4548173187259
最新バージョンのいずれかを選択してください：
Activating transcription factor 6 derepression mediates neuroprotection in Huntington disease
Naranjo J R, et al.
The Journal of Clinical Investigation, 126(2), 627-638 (2016)
Tomonori Kaneko et al.
Nature communications, 9(1), 4549-4549 (2018-11-02)
Src homology 2 (SH2) domains play a critical role in signal transduction in mammalian cells by binding to phosphorylated Tyr (pTyr). Apart from a few isolated cases in viruses, no functional SH2 domain has been identified to date in prokaryotes.
A A Ivanov et al.
Oncogene, 36(42), 5852-5860 (2017-06-20)
Mitogen-activated protein kinase kinase 3 (MKK3) is a dual threonine/tyrosine protein kinase that regulates inflammation, proliferation and apoptosis through specific phosphorylation and activation of the p38 mitogen-activated protein kinase. However, the role of MKK3 beyond p38-signaling remains elusive. Recently, we
Flavivirus NS3 and NS5 proteins interaction network: a high-throughput yeast two-hybrid screen
Le B M, et al.
BMC Microbiology, 11(1), 234-234 (2011)
Hiroshi Qadota et al.
Molecular biology of the cell, 27(10), 1606-1620 (2016-03-25)
UNC-89 is a giant polypeptide located at the sarcomeric M-line of Caenorhabditis elegans muscle. The human homologue is obscurin. To understand how UNC-89 is localized and functions, we have been identifying its binding partners. Screening a yeast two-hybrid library revealed
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