B3150

モノクロナール抗タンパク質A

clone SPA-27, purified immunoglobulin, buffered aqueous solution

• 別名: Monoclonal Anti-Protein A
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: biotin conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: SPA-27, monoclonal
• 形状: buffered aqueous solution
• テクニック: direct ELISA: 1:80,000-1:160,000
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Protein A is a cell wall associated protein isolated from Staphylococcus aureus Cowan 1 strain. The 42 kDa protein consists of a single polypeptide chain. Protein A possesses a specific affinity for Fc region of many mammalian immunoglobulins as human, rabbit, and guinea pig IgGs and therefore, considered as a universal reagent in biochemistry and immunology. Antibodies to protein A have been produced in rabbits, guinea pigs, chickens and mice. Protein A affinity chromatography has been achieved for the purification of immmunoglobulins. Monoclonal Anti-Protein A (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

特異性

プロテインAに特異的であり、プロテインGとの交差反応性は認められません。遊離プロテインAや、IgGに結合したプロテインAと反応し、プロテインAのFc結合活性には影響しません。

免疫原

黄色ブドウ球菌(Staphy­lococcus aureus)のCowan I 株由来プロテインA。

アプリケーション

Monoclonal Anti-Protein A-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:80,000 - 1:160,000.

生物化学的／生理学的作用

Protein A is an immunoglobulin-binding protein present on the surface of Staphylococcus aureus and is also secreted into the extracellular environment. It binds the Fc region of Igs and Fab regions of B cell receptors. The coupling of biotin to monoclonal Anti-Protein A antibody allows for the binding of various labels such as avidin or streptavidin.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

調製ノート

Prepared by conjugation of biotinamidocaproate N-hydroxysuccinimide ester to fractionated monoclonal anti-Protein A.

保管および安定性

Store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable