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由来生物
goat
品質水準
結合体
biotin conjugate
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
secondary antibodies
クローン
polyclonal
形状
buffered aqueous solution
化学種の反応性
mouse
濃度
≥2 mg/mL
テクニック
direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
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詳細
IgG is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γchains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.
アプリケーション
Anti-Mouse IgG (Fc specific)-Biotin antibody has been used in enzyme linked immunosorbent assay (ELISA), dot blot, immunohistology, nuclear labeling, ELISA-inhibition method. immunohistochemistry.
Anti-Mouse IgG (Fc specific)-Biotin antibody produced in goat was used for immunocytochemistry staining to visualize neonatal myosin in chicken muscle sections. It was used for ELISPOT analysis to study IgG levels in mice infected with cholera toxin.
生物化学的／生理学的作用
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Mouse IgG (Fc specific) antibody allows for the binding of various labels such as avidin or streptavidin.
その他情報
Antibody adsorbed with human IgG and rat serum proteins.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
調製ノート
バックグラウンド染色を低減するため、ヒトまたはラットサンプルで吸収済みです。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
B9904-2ML:
B9904-VAR:
B9904-.5ML:
B9904-BULK:
B9904-1ML:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Kate E Broderick et al.
Human vaccines, 7 Suppl, 22-28 (2011-01-26)
Electropermeabilization of mammalian cells is a technique that has been used for the delivery of therapeutics, such as DNA plasmids or DNA vaccines. Typically, delivery via electropermeabilization occurs through injection of the substance into the tissue of interest followed by
IgE Cross Reactivity Between Reindeer and Bovine Milk ss-Lactoglobulins in Cow's Milk Allergic Patients
Suutari T J, et al.
Journal of investigative medicine high impact case reports, 16(5), 296-296 (2006)
André Steinecke et al.
Development (Cambridge, England), 141(2), 460-471 (2014-01-02)
Inhibitory interneurons control the flow of information and synchronization in the cerebral cortex at the circuit level. During embryonic development, multiple subtypes of cortical interneurons are generated in different regions of the ventral telencephalon, such as the medial and caudal
Malika Guendouz et al.
Pharmaceutical biology, 55(1), 2145-2152 (2017-10-07)
Royal jelly (RJ) has long been used to promote human health. The current study investigated the preventive effects of RJ against the development of a systemic and intestinal immune response in mice allergic to cow's milk proteins. Balb/c mice treated
Angela Schippers et al.
Clinical immunology (Orlando, Fla.), 144(2), 87-97 (2012-06-20)
IgA production in the gut-associated lymphoid tissue represents a pivotal defense mechanism against luminal pathogens. The other important challenge for the GALT is the induction of local and systemic hyporesponsiveness (tolerance) to dietary antigens and luminal bacterial flora to prevent
資料
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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