D2916

λDNA酵素処理フラグメント

for DNA electrophoresis

• UNSPSCコード: 12352200
• NACRES: NA.25

特性

• 品質水準: 100; 200
• フォーム: buffered aqueous solution
• 使用法: 100 uses (0.1 mg size); 20 uses (20 μg size)
• 適合性: suitable for electrophoresis (DNA)
• 保管温度: −20°C

詳細

詳細

Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

アプリケーション

Lambda DNA Mixed Digest has been used for DNA electrophoresis.

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

構成

10 mM Tris-HCl溶液(pH 8.0、1 mM EDTA含有)

調製ノート

Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.

その他情報

For optimal resolution, the recommended agarose gel concentration is 0.4%.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable