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由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
HER2-96, monoclonal
形状
buffered aqueous solution
分子量
antigen 185 kDa (complete protein)
antigen 97-115 kDa (extracellular ligand-binding domain)
化学種の反応性
human
濃度
~2 mg/mL
テクニック
immunoprecipitation (IP): 0.5-1 μg/test using RIPA lysate of cultured CB2 cells (CHO cell line transfected with erbB-2)
indirect ELISA: suitable
microarray: suitable
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ERBB2(2064)
詳細
Monoclonal Anti-c-erbB-2 (HER-2, Neu) (mouse IgG1 isotype) is derived from the (human epidermal growth factor receptor 2) HER2-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified extracellular domain of human erbB-2, from transfected cells. The human erbB-2 oncogen (also known as HER2, HER-2 or neu) encodes a transmembrane growth factor orphan receptor with a M.W. of 185 kDa (p185). The complete protein consists of an internal cytoplasmic structure with tyrosine kinase activity, a short hydrophobic transmembrane section and an extracellular ligand-binding domain (ECD).
免疫原
トランスフェクション細胞由来ヒトerbB-2細胞外ドメインの精製物。
アプリケーション
Monoclonal Anti-c-erbB-2 antibody produced in mouse has been used in :
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
- immunohistochemistry
- studies on receptor internalization, phosphorylation, ligand binding and tumor growth inhibition
生物化学的／生理学的作用
Erb-b2 receptor tyrosine kinase 2 (ErbB-2) has a pivotal role in signal transduction, where it can stimulate the signal even in the absence of ligand by forming heterodimers with erbB-1/EGF-R, erbB-3, and erbB-4 and hence can modifies ligand affinity. Thus, erbB-2 alters the intracellular responses elicited by EGF and NDF. This control is due to the fact that erbB-2, when in complex with another erbB family receptor, decelerates the rate of ligand dissociation. Therefore, erbB-2 may act as a signalling subunit for other receptors rather than a true growth factor receptor. Because overexpression of the erbB-2 proto-oncogene is frequently associated with an aggressive clinical course of certain human adenocarcinomas, the encoded protein is an attractive target for immunotherapy. Indeed, antibodies to erbB-2 were found to block the crosstalk with growth factor receptors. In addition, receptor overexpression is considered a predictor of poor survival and short time of relapse.
物理的形状
0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
E2777-BULK:
E2777-VAR:
E2777-.2ML:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
HER2-Associated Radioresistance of Breast Cancer Stem Cells Isolated from HER2-Negative Breast Cancer Cells
Duru A, et al.
Clinical Cancer Research, 18(24), 6634-6647 (2012)
L N Klapper et al.
Oncogene, 14(17), 2099-2109 (1997-05-01)
ErbB-2 is an orphan receptor that belongs to a family of tyrosine kinase receptors for either epidermal growth factor (EGF) or Neu differentiation factor (NDF/neuregulin). Because overexpression of the erbB-2 proto-oncogene is frequently associated with an aggressive clinical course of
Götz Pilarczyk et al.
International journal of molecular sciences, 18(2) (2017-02-18)
In cancer, vulnerable breast epithelium malignance tendency correlates with number and activation of ErbB receptor tyrosine kinases. In the presented work, we observe ErbB receptors activated by irradiation-induced DNA injury or neuregulin- 1 β application, or alternatively, attenuated by a
Jekabs Krigerts et al.
Biophysical journal, 120(4), 711-724 (2021-01-17)
Finding out how cells prepare for fate change during differentiation commitment was our task. To address whether the constitutive pericentromere-associated domains (PADs) may be involved, we used a model system with known transcriptome data, MCF-7 breast cancer cells treated with
R Goldman et al.
Biochemistry, 29(50), 11024-11028 (1990-12-18)
The erbB-1 and erbB-2 protooncogenes encode homologous membrane receptors that respectively bind epidermal growth factor (EGF) and a still incompletely characterized ligand. Binding of EGF to its receptor is known to increase tyrosine phosphorylation of the erbB-2/neu receptor in tumor
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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