F5512

Anti-Human IgG (Fab specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• 別名: Goat Anti-Human IgG (Fab specific)−Fluorescein isothiocyanate
• MDL番号: MFCD00162433
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: goat
• 品質水準: 200
• 結合体: FITC conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 保管条件: protect from light
• テクニック: immunofluorescence: 1:16-1:32 using Hep2 cells
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

全てのヒトイムノグロブリンに結合します。
マウスまたはラットサンプルのバックグラウンドを低減します。

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

アプリケーション

Anti-Human IgG (Fab specific)-FITC antibody has been used:

• in fluorescent staining
• in dual color fluorescent labeling
• in fluorescence-activated cell sorting (FACS) analysis

Anti-Human IgG (Fab specific)-FITC antibody produced in goat may be used at a working antibody dilution of 1:16 - 1:32 for anti-nuclear antibody (A.N.A.) assay on acetone-fixed human Hep2 cells. This antibody was used in FACS of HNE2 and KNE2/LMP1 cells. It was also used to perform TARDIS assay for detection of topoisomerases I and II that are covalently bound to DNA in K562 leukemia cells.

生物化学的／生理学的作用

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti- Human IgG (Fab specific) antibody allows for the visualization of protein by fluorescent microscopy.

その他情報

マウスおよびラットIgGとの交差反応性はあリません。

物理的形状

0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)