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詳細
The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.
アプリケーション
Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.
Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.
生物化学的/生理学的作用
Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin.
その他情報
Crude luciferin and luciferase
再構成
25 mLの水で再構成すると、バイアルの内容物は0.06 Mリン酸カリウム、pH 7.4、0.02 M MgSO4になります。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
FLE250-1VL:
FLE250-BULK:
FLE250-VAR:
Involvement of acetylcholinesterase and protein kinase C in the protective effect of caffeine against beta-amyloid-induced alterations in red blood cells
Biochimie, 121, 52-59 (2016)
Distribution patterns of organic pollutants and microbial processes in marine sediments across a gradient of anthropogenic impact
Environmental Pollution (Barking, Essex : 1987), 242, 1860-1870 (2018)
Concentrations and uptake of dissolved organic phosphorus compounds in the Baltic Sea
Frontiers in Marine Science, 5(386) (2018)
Microbial responses to polycyclic aromatic hydrocarbon contamination in temporary river sediments: Experimental insights
The Science of the Total Environment, 541, 1364-1371 (2016)
American journal of physiology. Regulatory, integrative and comparative physiology, 308(5), R411-R418 (2015-01-02)
The circulating erythrocyte, by virtue of the regulated release of ATP in response to reduced oxygen (O2) tension, plays a key role in maintaining appropriate perfusion distribution to meet tissue needs. Erythrocytes from individuals with Type 2 diabetes (DM2) fail
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