G3042
Anti-GAL4 DNA-BD antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
別名:
Anti-Galactose 4 DNA Binding Domain
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About This Item
結合体:
unconjugated
application:
WB
クローン:
polyclonal
テクニック:
western blot: 1-2 μg/mL using GAL4 (DBD 1-147) fusion protein
citations:
12
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由来生物
rabbit
品質水準
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
フォーム
buffered aqueous solution
テクニック
western blot: 1-2 μg/mL using GAL4 (DBD 1-147) fusion protein
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
詳細
The polyclonal Anti-GAL4 DNA-BD rabbit antibody is a synthetic peptide that recognizes GAL4 DNA binding domain fusion proteins. GAL4 protein is an 881 amino acid transcription factor. It contains functionally independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a constituent of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey).
特異性
Anti-GAL4 DNA-BD recognizes GAL4 DNA binding domain fusion proteins.
免疫原
Saccharomyces cerevisiaeのGAL4タンパク質DNA結合ドメイン(アミノ酸39~52)に相当する合成ペプチド。
アプリケーション
Anti-GAL4 DNA-BD antibody produced in rabbit has been used in western blotting.
生物化学的／生理学的作用
GAL4 transcription factor is involved in the induction of genes that regulate galactose metabolism in Saccharomyces cerevisiae. The yeast two hybrid system is widely used for the characterization of domains that are necessary and sufficient for the interaction of two known proteins by deletion and mutational analysis.
物理的形状
0.01 M PBS溶液(pH 7.4, 1%BSA, 15 mMアジ化ナトリウム含有)。
保管および安定性
For continuous use store at 2-8°C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
G3042-BULK:
G3042-200UG:
G3042-VAR:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
Sirtuin 7-dependent deacetylation of DDB1 regulates the expression of nuclear receptor TR4
Karim Mdf, et al.
Biochemical and biophysical research communications, 490(2), 423-428 (2017)
Anil K Panigrahi et al.
Proceedings of the National Academy of Sciences of the United States of America, 120(4), e2216436120-e2216436120 (2023-01-20)
Enhancers not only activate target promoters to stimulate messenger RNA (mRNA) synthesis, but they themselves also undergo transcription to produce enhancer RNAs (eRNAs), the significance of which is not well understood. Transcription at the participating enhancer-promoter pair appears coordinated, but
Alexandre Nore et al.
Nature communications, 13(1), 7048-7048 (2022-11-18)
Meiosis requires the formation of programmed DNA double strand breaks (DSBs), essential for fertility and for generating genetic diversity. DSBs are induced by the catalytic activity of the TOPOVIL complex formed by SPO11 and TOPOVIBL. To ensure genomic integrity, DNA
SIRT7 regulates the nuclear export of NF-$\kappa$B p65 by deacetylating Ran
Sobuz SU, et al.
Biochimica et biophysica acta. General subjects, 1866(9), 1355-1367 (2019)
Mitsuki Uemura et al.
Veterinary and comparative oncology, 18(2), 247-255 (2019-09-14)
RAD51 forms a complex with BRCA2 and plays a central role in the DNA damage response pathway that is associated with homologous recombination. The structures of RAD51 and its homologues are highly conserved from prokaryotes to higher eukaryotes. Although a
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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