G7914

Anti-Glycogen Synthase Kinase-3β (GSK-3β) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• MDL番号: MFCD01633410
• UNSPSCコード: 12352203
• NACRES: NA.44
• 結合体: unconjugated
• application: ARR; WB
• クローン: polyclonal
• 化学種の反応性: human, rat
• citations: 15
• テクニック: microarray: suitable; western blot: 1:2,000 using rat brain extract

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 46 kDa
• 化学種の反応性: human, rat
• テクニック: microarray: suitable; western blot: 1:2,000 using rat brain extract
• UniProtアクセッション番号: P49841
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GSK3B(2932) ; rat ... Gsk3b(84027)

詳細

詳細

Anti-Glycogen Synthase Kinase-3β (GSK-3β) is developed in rabbit using a synthetic peptide (KGDRGQTNNAASASASNST) corresponding to the C-terminal region of human GSK-3β conjugated to KLH as immunogen. Glycogen Synthase Kinase-3β (GSK-3β) is present in cytoplasm, nucleus and mitochondria. GSK-3 exists as two closely related protein kinases, termed GSK-3α and GSK-3β. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids. It shows a wide range of tissue expression with the highest expression in brain.

Glycogen synthase kinase-3 β (GSK3β) is a 47kD protein that belongs to serine/ threonine protein kinase family and is expressed ubiquitously as a constitutively active kinase. It has a pivotal role in regulating the degradation of β-catenin by the tumor suppressor gene product APC. Anti-Glycogen Synthase Kinase-3β (GSK-3β) antibody can be used in microarray. Rabbit anti- GSK3β antibody reacts specifically with rat GSK3β but not with GSK3α.

免疫原

Synthetic peptide corresponding to amino acids 403-420 of human glycogen synthase kinase 3 β (GSK3β), coupled to KLH. It is identical in rat and highly conserved in frog and zebrafish GSK3β.

アプリケーション

Anti-Glycogen Synthase Kinase-3β (GSK-3β) antibody produced in rabbit has been used in immunoblotting assays for analysis of protein expression.

生物化学的／生理学的作用

Glycogen synthase kinase-3 (GSK-3) specifically phosphorylates glycogen synthase, a critical enzyme regulating glucose storage, and mediates insulin regulation of glycogen synthesis.(8) GSK-3β phosphorylates regulatory sites in the central region of APC. Binding of β-catenin to APC, marking β-catenin for destruction, is dependent on phosphorylation of APC by GSK-3β. Thus, GSK-3β may play a critical role in regulating β-catenin stability during development and tumor progression. It is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues.

物理的形状

0.01 M PBS溶液(pH 7.4, 1% BSA, 15 mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable