H9539

抗HMGB1 (HMG1) (C-末端) ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: Anti-Amphoterin, Anti-High mobility group box 1, Anti-High mobility group protein 1
• MDL番号: MFCD02263062
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 25 kDa
• 化学種の反応性: rat, mouse, human
• 包装: antibody small pack of 25 μL
• 強化検証: independent; Learn more about Antibody Enhanced Validation
• テクニック: immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates.; indirect immunofluorescence: suitable; western blot: 0.5-1 μg/mL using 3T3 or PC12 cell lysates.
• UniProtアクセッション番号: P09429
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HMGB1(3146) ; mouse ... Hmgb1(15289) ; rat ... Hmgb1(25459)

詳細

詳細

The High Mobility Group (HMG1) is secreted by activated monocytes and macrophages. The gene is mapped to human chromosome 13q12.

免疫原

ヒトHMGB1のアミノ酸165~180に相当する合成ペプチドのKLH結合体。この配列はマウスおよびラットで保存されています。

アプリケーション

Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit has been used in ELISA and immunofluorescence.

生物化学的／生理学的作用

In the nucleus, HMGB1 (high-mobility group protein B1) works as a nucleosome stabilizer and transcriptional regulator. It also exhibits chaperone-like activity and suppresses aggregation of polyglutamine. It has a role in inflammation and autophagy. HMGB1 can interact with toll-like receptor (TLR) 2 and 4, interleukin-1β, chemokine (C-X-C motif) ligand 12 (CXCL12) and pathogen-associated molecular pattern (PAMP) molecules. It also works as a damage-associated molecular pattern molecule (DAMP).

物理的形状

15 mMアジ化ナトリウム含有のpH 7.4の0.01 Mリン酸緩衝食塩水溶液.

保管および安定性

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

その他情報

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)