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アプリケーション
(D-N-アセチルグルコサミン)2またはN-アセチルノイラミン酸含有糖タンパク質の精製に使用できます。
生物化学的/生理学的作用
WGAは血液型特異的ではありませんが、N-アセチル-β-D-グルコサミニル残基およびN-アセチル-β-D-グルコサミンオリゴマ-に対する親和性を有しています。WGAには、タンパク質に結合した糖質が含まれません。
物理的形状
0.9%NaCl中の15 mMアジ化ナトリウム懸濁液。
調製ノート
コンジュゲ-トはアフィニティ-精製したレクチンから調製されます。
保管分類コード
10 - Combustible liquids
WGK
WGK 3
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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PloS one, 7(5), e34274-e34274 (2012-05-17)
There is controversy with respect to molecular characteristics of insulin analogues. We report a series of experiments forming a comprehensive characterisation of the long acting insulin analogues, glargine and detemir, in comparison with human insulin, IGF-1, and the super-mitogenic insulin
The Journal of biological chemistry, 291(8), 3905-3917 (2015-12-20)
The normal cellular prion protein (PrP) is a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein. However, in pancreatic ductal adenocarcinoma cell lines, such as BxPC-3, PrP exists as a pro-PrP retaining its glycosylphosphatidylinositol (GPI) peptide signaling sequence. Here, we report the identification
International journal of molecular sciences, 24(2) (2023-01-22)
In Alzheimer's disease (AD), the reduction in acetylcholinesterase (AChE) enzymatic activity is not paralleled with changes in its protein levels, suggesting the presence of a considerable enzymatically inactive pool in the brain. In the present study, we validated previous findings
The Biochemical journal, 270(1), 57-61 (1990-08-15)
Chromaffin-granule membranes were separated into insoluble and soluble fractions after extraction with lithium di-iodosalicylate (LDIS). These fractions were characterized by one- and two-dimensional gel electrophoresis, and glycoproteins were detected after electroblotting with peroxidase-labelled concanavalin A and wheat-germ agglutinin (WGA). The
Communications biology, 4(1), 161-161 (2021-02-07)
Mutations in CLN3 lead to photoreceptor cell loss in CLN3 disease, a lysosomal storage disorder characterized by childhood-onset vision loss, neurological impairment, and premature death. However, how CLN3 mutations cause photoreceptor cell death is not known. Here, we show that
資料
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
プロトコル
This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.
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