M0691

モノクロナール抗ミオシンVI マウス宿主抗体

~1 mg/mL, clone MUD-19, purified from hybridoma cell culture

• 別名: Anti-DFNA22, Anti-DFNB37
• MDL番号: MFCD02263784
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: MUD-19, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~150 kDa by SDS-PAGE
• 化学種の反応性: human, mouse, rat
• 濃度: ~1 mg/mL
• テクニック: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 2 μg/mL using A431 total cell extract
• アイソタイプ: IgG1
• UniProtアクセッション番号: Q9UM54
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MYO6(4646) ; mouse ... Myo6(17920) ; rat ... Myo6(315840)

詳細

詳細

Monoclonal Anti-Myosin VI (mouse IgG1 isotype) is derived from the MUD-19 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide corresponding to amino acids of human myosin VI. Myosin VI is a relatively abundant widespread unconventional myosin composed of an N-terminal motor domain, a light chain binding neck region, a coil-coiled region and a highly conserved C-terminal domain.

Myosins are actin-dependent motors that have 15 classes based on their phylogenetics . Myosin VI is an unconventional actin motor that moves towards the minus ends of actin filaments, which is opposite to the movement of conventional myosins.

特異性

Monoclonal Anti-Myosin VI antibody is specific for myosin VI in rats, humans and mice.

アプリケーション

Monoclonal Anti-Myosin VI antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation
• western blot assay
• immunohistochemistry

生物化学的／生理学的作用

Myosin VI is involved in the generation of cell shape change, cell motility, membrane remodeling and possibly in organelle and particle transport or tethering. It is also involved in membrane trafficking pathways in cultured mammalian cells where it is associated with the membrane ruffles and the trans-Golgi network. Myosin VI plays an essential role in the development of the mouse inner ear where it is expressed in the hair cell body, in the actin-rich cuticular plate at the base of the stereocilia and in the peri cuticular necklace region. Myosin VI gene mutations are involved in deafness and balance defects in the Snell′s Waltzer mouse. Myosin VI is present in the Golgi complex and may regulate vesicular transport across the membranes.

物理的形状

0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable