MAK521

Fumarase Assay Kit

sufficient for 100 colorimetric tests

• UNSPSCコード: 12161503
• NACRES: NA.84

特性

• 品質水準: 100
• 検出方法: colorimetric
• 関連疾患: neurological disorders; neonatal diseases
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Fumarase (or fumarate hydratase) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate. Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc.

特徴および利点

• Linear detection range: 0.4 to 70 U/L
• Compatible with high-throughput handling systems.

適合性

The kit is suitable for fumarase activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)

原理

The Fumarase Assay Kit is a non-radioactive, colorimetric assay based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

安全性情報

• ピクトグラム: GHS02,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H225,H290,H315,H319,H336
• 注意書き: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Central nervous system
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 3