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Merck
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安全性情報

MBD0005

Sigma-Aldrich

Microbial DNA standard from Salmonella enterica

Suitable for PCR, sequencing and NGS, 10 ng/μL

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About This Item

UNSPSCコード:
41105500
NACRES:
NA.24

品質水準

フォーム

liquid

濃度

10 ng/μL

テクニック

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

輸送温度

ambient

保管温度

−20°C

詳細

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Salmonella enterica genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.
Salmonella enterica serotype Typhimurium (also S. Typhimurium) is a rod-shaped gram-negative bacterium of the family Enterobacteriaceae. It is an important food-borne pathogen that in humans causes intestinal inflammation, diarrhea, and the presence of neutrophils in stool samples.2 Growth of S. enterica in the gut lumen causes depletion of the healthy microbial community.3 Infection with Salmonella can induce inflammation and increase the risk for inflammatory bowel disease (IBD)4

Read here how to use our standards to ensure data integrity for your microbiome research.

アプリケーション

Suitable for Quantitative standard for PCR, Sequencing and NGS

特徴および利点

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

物理的形状

Liquid -The genomic DNA is provided at ≥10 ng/μl concentration in TE buffer pH 8.0

その他情報

Strain: Salmonella enterica subsp. enterica serovar Typhimurium strain

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MBD0005-VAR:
MBD0005-0.3UG:
MBD0005-BULK:
MBD0005-0.3UG-PW-P:
MBD0005-0.3UG-PW:


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文書ライブラリにアクセスする

Kim O Gradel et al.
Gastroenterology, 137(2), 495-501 (2009-04-14)
Various commensal enteric and potentially pathogenic bacteria may be involved in the pathogenesis of inflammatory bowel diseases (IBD). We compared the risk of IBD between a cohort of patients with documented Salmonella or Campylobacter gastroenteritis and an age- and gender-matched
Inna Sekirov et al.
Gut microbes, 1(1), 30-41 (2011-02-18)
Gastrointestinal infections involve an interactive tripartite relationship between the invading pathogen, the host, and the host's resident intestinal microbiota. To characterize the host inflammatory response and microbiota alterations during enteric salmonellosis, C57BL/6 mice were pre-treated with a low dose of
Fecal leukocytes in diarrheal illness.
J C Harris et al.
Annals of internal medicine, 76(5), 697-703 (1972-05-01)
J Paul Brooks et al.
BMC microbiology, 15, 66-66 (2015-04-17)
Characterizing microbial communities via next-generation sequencing is subject to a number of pitfalls involving sample processing. The observed community composition can be a severe distortion of the quantities of bacteria actually present in the microbiome, hampering analysis and threatening the

資料

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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