P5872

モノクロナール抗リン酸化チロシン

clone PT-66, purified from hybridoma cell culture

• 別名: モノクロナール抗リン酸化チロシン
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: PT-66, monoclonal
• 形状: buffered aqueous solution
• 包装: antibody small pack of 25 μL
• 濃度: ~2 mg/mL
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: 0.5-1.0 μg/mL using phosphotyrosine conjugated to BSA; radioimmunoassay: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of human platelets
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

ELISA, 競合ELISAにおいて、本抗体は、ホスホチロシンの遊離アミノ酸型および、BSA・KLH等のキャリア結合型の両方と特異的に反応します。非リン酸化チロシン、ホスホスレオニン、ホスホセリン、AMP、ATPとの交差反応はみられません。

Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the PT-66 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with phosphotyrosine-BSA conjugate.

特異性

Monoclonal Anti-Phosphotyrosine is specific for phosphorylated tyrosine both as the free amino acid or when conjugated to carriers such as BSA or KLH. It does not react with non-phosphorylated tyrosine or other phosphorylated amino acids, including serine

免疫原

BSA結合ホスホチロシン

アプリケーション

Monoclonal Anti-Phosphotyrosine has been used in

• immunoblotting,
• immunofluorescence,
• immunohistochemistry
• immunocytochemistry
• flow cytometry
• immunoprecipitation
• enzyme linked immunosorbent assay (ELISA)
• radio immunoassay (RIA)
• immunoaffinity isolation

生物化学的／生理学的作用

Protein phosphorylation is a basic signaling mechanism that modifies protein function in eukaryotic cells. Serine, threonine, and tyrosine are the major phosphorylated amino acids in proteins. Tyrosine phosphorylation is a rare post-translational event in normal tissues, accounting for only 0.03% of phosphorylated amino acids. However, this phosphorylation increases several folds by various activation signals and the process is mediated by protein tyrosine kinases. Protein-tyrosine kinases (PTKs) are enzymes that catalyze the transfer of γ-phosphate of ATP to tyrosine residues of protein substrates. The PTKs are responsible for many biological processes like cell cycle, proliferation, oncogenesis, and development.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable