SAB4200047

Anti-BRD7 antibody, Rat monoclonal

clone BRM 2D3, purified from hybridoma cell culture

• 別名: Anti-BP75, Anti-Bromodomain containing 7, Anti-CELTIX1, Anti-NAG4, Monoclonal Anti-BRD7 antibody produced in rat
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ICC; IP; WB
• クローン: BRM 2D3, monoclonal
• 化学種の反応性: rat, mouse, monkey, human, bovine, canine
• citations: 4
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1.0 μg/mL using HeLa cells extract

特性

• 由来生物: rat
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: BRM 2D3, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~80 kDa
• 化学種の反応性: rat, mouse, monkey, human, bovine, canine
• 包装: antibody small pack of 25 μL
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1.0 μg/mL using HeLa cells extract
• アイソタイプ: IgG2a
• UniProtアクセッション番号: Q9NPI1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... BRD7(29117)

詳細

詳細

Monoclonal Anti-BRD7 (rat IgG2a isotype) is derived from the hybridoma BRM 2D3 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a fusion protein expressing a fragment of human BRD7. Bromodomain containing (BRD7) is a novel bromodomain gene.

アプリケーション

Monoclonal Anti-BRD7 antibody produced in rat has been used in several immunochemical techniques including

• immunoblotting
• immunoprecipitation
• immunocytochemistry

生物化学的／生理学的作用

Bromodomain containing (BRD7) inhibits cell growth and cell cycle progression by transcriptional regulation of some cell cycle-related genes as well as some important molecules involved in ras/mitogen-activated protein kinase(MEK)/extracellular-signal-regulated kinase (ERK) and retinoblastoma tumor suppressor protein (RB)/ E2 factor (E2F) pathways. Its transcriptional down regulation has been shown to be critical to the pathogenesis of nasopharyngeal carcinoma (NPC). Specifically, DNA methylation decreases the expression of BRD7 in NPC cells. Furthermore, DNA methylation of BRD7 promoter is increased in the tumor and in matched blood samples from NPC patients than in blood samples from healthy individuals. Therefore, it has been suggested that DNA methylation of BRD7 promoter might act as a potential diagnostic marker in NPC.

物理的形状

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable