SAB4200232

Anti-GAD2(GAD67) Antibody

mouse monoclonal, N-GAD65

• 別名: Anti-GAD2, Anti-glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Anti-glutamate decarboxylase 65 kDa isoform
• MDL番号: MFCD03455043
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: IHC; IP; WB
• クローン: N-GAD65, monoclonal
• 化学種の反応性: human, mouse, monkey, rat
• citations: 12
• テクニック: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.25-0.5 μg/mL using whole extracts of rat brain

特性

• 製品名: Anti-GAD65 antibody , Mouse monoclonal, clone N-GAD65, purified from hybridoma cell culture
• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: N-GAD65, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~65 kDa
• 化学種の反応性: human, mouse, monkey, rat
• 濃度: ~1.0 mg/mL
• テクニック: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.25-0.5 μg/mL using whole extracts of rat brain
• アイソタイプ: IgG1
• UniProtアクセッション番号: Q05329
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GAD2(2572) ; mouse ... Gad2(14417) ; rat ... Gad2(24380)

詳細

詳細

Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain.

アプリケーション

Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA.

生物化学的／生理学的作用

Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable