SAB4200399

Anti-WIPI-2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 別名: Anti-ATG18B, Anti-Atg21, Anti-CGI-50, Anti-WIPI2, WD repeat domain, phosphoinositide interacting 2
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~49 kDa
• 化学種の反応性: rat, mouse, human
• 濃度: ~1.0 mg/mL
• テクニック: immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells.; western blot: 1-2 μg/mL using using whole extracts of HEK-293T cells over-expressing mouse WIPI-2.
• UniProtアクセッション番号: Q9Y4P8
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... WIPI2(26100) ; mouse ... Wipi2(74781) ; rat ... Wipi2(288498)

詳細

詳細

The WIPI2 gene is mapped on the human chromosome at 7p22.1. WIPI2 has a 7-bladed propeller structure and contains a conserved motif.

WD repeat domain, phosphoinositide-interacting 2 (WIPI-2) belongs to the WIPI protein family. It is localized on the autophagy membrane and plasma membrane. Apart from this, it is also present on the membranes near the Golgi cisternae. WIPI2 is a mammalian ortholog of Atg18, which is ubiquitously expressed in a variety of cell lines.

特異性

Anti-WIPI-2 recognizes human, rat, and mouse WIPI-2.

アプリケーション

Anti-WIPI-2 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

生物化学的／生理学的作用

WIPI-2 (WD repeat domain, phosphoinositide-interacting 2) plays a vital role in the transformation of omegasomes into autophagosomes. The above protein functions as a mammalian effector of phosphatidylinositol 3-phosphate (PtdIns3P). WIPI2 aids recombinant capsid protein VP1 (rVP1) in upregulating autophagy, MAPK1 (Mitogen-activated protein kinase 1)/3 phosphorylation and matrix metallopeptidase 9 (MMP-9) activity, which are required for macrophage migration.

物理的形状

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable