SAB4200714

Anti-Heat Shock Protein 70 (HSP70) antibody, Mouse monoclonal

clone BRM-22, purified from hybridoma cell culture

• 別名: Anti-HSP70, Anti-HSPA1A
• UNSPSCコード: 12352203
• NACRES: NA.41
• クローン: BRM-22, monoclonal
• application: DB; ELISA; IF; IHC
• 化学種の反応性: plant, bovine, rabbit, chicken, human, fish, insect, monkey, dog, rat, sea urchin, mouse
• テクニック: ELISA: suitable; dot blot: suitable; immunoblotting: 0.25-0.5 μg/mL using HeLa cell line extract; immunofluorescence: suitable; immunohistochemistry: suitable
• citations: 14

特性

• 抗体製品の状態: purified from hybridoma cell culture
• 品質水準: 200
• 抗体製品タイプ: primary antibodies
• クローン: BRM-22, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~70 kDa
• 化学種の反応性: plant, bovine, rabbit, chicken, human, fish, insect, monkey, dog, rat, sea urchin, mouse
• 濃度: ~1 mg/mL
• テクニック: ELISA: suitable; dot blot: suitable; immunoblotting: 0.25-0.5 μg/mL using HeLa cell line extract; immunofluorescence: suitable; immunohistochemistry: suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: P0DMV8
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HSPA1A(3303) ; mouse ... HSPA1A(193740) ; rat ... HSPA1A(24472)

詳細

詳細

Anti-heat shock protein 70 (HSP70) antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the BRM-22 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with purified bovine brain HSP70. Monoclonal Anti-heat shock protein 70 (HSP70) specifically recognizes HSP70 from bovine, mouse, monkey, dog, human, rat, chicken,octopus, insects, plant, fish, rabbit and sea urchin origin.
HSP70 / heat shock protein family A (Hsp70) member 1A (HSPA1A), is encoded by the gene mapped to human chromosome 6p21.33. It is widely expressed in all tissues. The encoded protein is characterized with an N-terminal ATPase domain of 45kDa and a C-terminal substrate binding domain of 25kDa which is further subdivided into a β-sandwich subdomain of 15kDa and a C-terminal α-helical subdomain.

免疫原

purified bovine brain HSP70

生物化学的／生理学的作用

The members of Hsp70 protein family are implicated in various cellular processes and are required for the response to environmental changes and survival. These proteins are essential constituents of the cellular network of molecular chaperones and folding catalysts. Hsp70 plays a vital role in several folding processes, such as the folding and assembly of newly synthesized proteins, refolding of misfolded and aggregated proteins. In addition, it also facilitates membrane translocation of organellar and secretory proteins and regulate the activity of regulatory proteins. Aberrations in the gene expression leads to skin color diversity.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

その他情報

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable