SRP0691

PI3K (P85 ALPHA) Protein

recombinant

• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• リコンビナント: expressed in baculovirus infected Sf9 cells
• アッセイ: 70-95% (SDS-PAGE)
• 形状: frozen liquid
• 分子量: ~86 kDa
• 濃度: 0.2 μg/μL
• アクセッション番号: NM_181523
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... PI3K (5295)

詳細

アプリケーション

Useful for Western blot and kinase assays.

生物化学的／生理学的作用

Recombinant full-length human PI3K (p85alpha) was expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. p85α modulates the interaction between PI3K and platelet-derived growth factor receptor ⁽¹⁾. Furthermore, estrogen receptor isoform ER-alpha binds in a ligand-dependent manner to the p85-alpha regulatory subunit of PI3K. Stimulation with estrogen increases ER-alpha-associated PI3K activity, leading to the activation of protein kinase B/AKT and endothelial nitric oxide synthase (eNOS) ⁽²⁾.

再構成

Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

保管および安定性

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

安全性情報

• ピクトグラム: GHS08,GHS07
• シグナルワード: Danger
• 危険有害性情報: H315,H319,H360D
• 注意書き: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危険有害性の分類: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable