G005644

HPLC Analysis of Nucleosides on Ascentis^® Express OH5 (HILIC Mode)

application for HPLC

特性

• テクニック: HPLC: suitable
• 試験パラメータ: column: Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U); mobile phase: [A] 5 mM ammonium acetate, pH 5.0 with acetic acid in 95:5, acetonitrile:water; [B] 5 mM ammonium acetate, pH 5.0 with acetic acid in 80:20, acetonitrile:water; gradient: 0% B held for 1 min; to 100% B in 10 min; held at 100% B for 1 min; flow rate: 0.3 mL/min; column temp.: 25 °C; detector: UV, 250 nm; injection: 2 μL; sample: 10 - 100 μg/mL in 95:5, acetonitrile:water (pH of buffer stock (in water) was adjusted before further dilution with water and/or acentonitrile)
• 適合性: application for HPLC
• アプリケーション: life science and biopharma

詳細

アナリシスノート

Nucleosides are involved in many aspects of metabolism. Analogs of nucleosides have medical application in antivirals or anticancer treatments. This application demonstrates the utility of hydrophilic interaction chromatography (HILIC) for the retention and resolution of nucleosides and analogs thereof. Elution is not solely explained by logD but by other ionic and solvophobic effects.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

アナライト

5-Methyluridine
DMT-2′O-TC-rU Phosphoramidite
2-Thiocytidine dihydrate
2′-O-Methylcytidine
Inosine
5-Methylcytidine
Cytidine
DMT-2′O-TC-rG(ib) Phosphoramidite
1-Methyladenosine
7-Methylguanosine
すべて表示 (10)