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일반 설명
Polyethylene glycol (PEG) is a hydrophilic polymer. It can be easily synthesized by the anionic ring opening polymerization of ethylene oxide, into a range molecular weights and variety of end groups. When crosslinked into networks PEG can have high water content, forming “hydrogels PEG is a suitable material for biological applications because it does not trigger an immune response.
애플리케이션
Polyethylene glycol (PEG) platelets were used:
- in PEG-directed gene delivery of protoplast transfection and incubation
- to prepare extraction for measuring cytosolic phosphoenolpyruvate carboxykinase (PEPCK) from plant tissues
- as DNA transformation agent in a study of transient gene expression of plant protoplasts, derived from rice leaf sheaths.
- in preparing isothermal assembly buffer and TSS broth preparation for unique nucleotide sequence (UNS)-guided assembly of repetitive DNA parts
- in transformation of DNA into log-phase cells of the budding yeast Saccharomyces cerevisiae.
- as DNA transformation reagent
기타 정보
Polymer networks with grafted cell adhesion peptides for highly biospecific cell adhesive substrates
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
Oleg Raitskin et al.
PloS one, 14(2), e0211598-e0211598 (2019-02-28)
Molecular tools adapted from bacterial CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats) systems for adaptive immunity have become widely used for plant genome engineering, both to investigate gene functions and to engineer desirable traits. A number of different Cas (CRISPR-associated)
Shen, Jinbo, et al. "Isolation, culture, and transient transformation of plant protoplasts
Shen J, et al.
Current Protocols in Cell Biology, 2014, 2-8 null
A simple and effective method to encapsulate tobacco mesophyll protoplasts to maintain cell viability
Lei R, et al.
Methods, 24-32 (2015)
Jennifer DeMars Tripp et al.
Yeast (Chichester, England), 30(5), 191-200 (2013-03-14)
Chemical-based methods have been developed for transformation of DNA into log-phase cells of the budding yeast Saccharomyces cerevisiae with high efficiency. Transformation of early stationary-phase cells, e.g. cells grown in overnight liquid cultures or as colonies on plates, is less
P D Drumheller et al.
Analytical biochemistry, 222(2), 380-388 (1994-11-01)
Polymer networks of poly(ethylene glycol) (PEG) in densely cross-linked matrices of acrylic acid (AA) and trimethylolpropane triacrylate were synthesized as biospecific cell adhesive substrates. Networks grafted with synthetic adhesion peptides produced substrates to investigate long-term, receptor-mediated cell/surface interactions, without nonspecific
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