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Merck
모든 사진(1)

주요 문서

935395

Sigma-Aldrich

N-(3-Azidopropyl)biotinamide

≥95%

동의어(들):

(3aS,4S,6aR)-N-(3-Azidopropyl)hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide, 1H-Thieno[3,4-d]imidazole-4-pentanamide, N-(3-azidopropyl)hexahydro-2-oxo-, (3aS,4S,6aR)-, N-(3-Azidopropyl)biotinamide

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About This Item

실험식(Hill 표기법):
C13H22N6O2S
CAS Number:
Molecular Weight:
326.42
MDL number:
UNSPSC 코드:
12352125
NACRES:
NA.21

Quality Level

분석

≥95%

형태

solid

색상

white to beige

저장 온도

2-8°C

SMILES string

[N-]=[N+]=NCCCNC(=O)CCCCC1SCC2NC(=O)NC12

InChI

InChI=1S/C13H22N6O2S/c14-19-16-7-3-6-15-11(20)5-2-1-4-10-12-9(8-22-10)17-13(21)18-12/h9-10,12H,1-8H2,(H,15,20)(H2,17,18,21)/t9-,10-,12-/m0/s1

애플리케이션

This reagent enables the specific labeling of various alkynylated molecules, such as DNA, oligonucleotides, and proteins, with biotin. The binding of biotin to avidin or streptavidin can be employed in downstream affinity applications, such as the isolation of biotinylated molecules or their interaction with streptavidin conjugates. Biotin azide undergoes a copper-catalyzed click reaction with terminal alkynes, enabling the incorporation of biotin and biotin derivatives into biomolecules that contain alkyne groups through azide-alkyne cycloaddition.

특징 및 장점

Biotin-azide (N-(3-Azidopropyl)biotinamide) is an azido derived biotin probe. Biotin-azide can be used to prepare various biotinylated conjugates via Click Chemistry.The conjugation of biotin and its derivatives to various biomolecules can be achieved through the widely recognized click chemistry methodology, followed by their detection using streptavidin, avidin, or NeutrAvidin biotin-binding proteins. Biotin azide serves as a valuable reagent for the synthesis of diverse biotinylated conjugates via Click Chemistry

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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