04-1585

Anti-IL-18 Antibody, clone 12E7.1

clone 12E7.1, from mouse

• 동의어(들): interleukin 18 (interferon-gamma-inducing factor), IL-1 gamma, IFN-gamma-inducing factor, iboctadekin, interleukin-18, Interleukin-1 gamma, Iboctadekin, Interferon gamma-inducing factor
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 12E7.1, monoclonal
• 종 반응성: human, mouse
• 기술: flow cytometry: suitable; immunohistochemistry: suitable
• 동형: IgMκ
• NCBI 수납 번호: NP_001553
• UniProt 수납 번호: Q14116
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... IL18(3606) ; mouse ... Il18(16173)

설명

일반 설명

Interleukin-18 (UniProt: Q14116; also known as IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma) is encoded by the IL18 (also known as IGIF, IL1F4) gene (Gene ID: 3606) in human. IL-18 is a pro-inflammatory cytokine that is shown to evoke both innate and acquired responses. It is synthesized with a propeptide (aa 1-36), which is subsequently cleaved off by caspases 1 and 4 to generate the active mature form. IL-18 is reported to be constitutively expressed in monocytes, macrophages, epithelial cells, osteoblasts, keratinocytes, and synovial fibroblasts. Its expression has also been reported in adrenal cortical cells. Mature IL-18 acts by binding to the IL-18 receptor α (IL-18α) to form a low affinity binary complex that recruits IL-18β to form a high affinity complex that leads to the activation of NF-kB and MAP kinase pathways. IL-18 can synergize with IL-12 to induce interferon-γ synthesis from T-helper 1 and natural killer cells.

면역원

KLH-conjugated linear peptide corresponding to human IL-18.

애플리케이션

Anti-IL-18 Antibody, clone 12E7.1 is an antibody against IL-18 for use in IH, FC.

Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in human tonsil tissue.

Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in inflamed skin and normal human spleen tissues.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

품질

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 1 g of this antibody detected IL-18 in mouse splenocytes.

표적 설명

~24 kDa observed

물리적 형태

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Mouse splenocytes

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable