04-824

Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal

culture supernatant, clone 68-4, from rabbit

• 동의어(들): Estradiol receptor, Nuclear receptor subfamily, estrogen receptor 1, estrogen receptor alpha
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: culture supernatant
• 항체 생산 유형: primary antibodies
• 클론: 68-4, monoclonal
• 종 반응성: mouse, rat, human
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NM_001437
• UniProt 수납 번호: P03372
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ESR2(2100) ; mouse ... Esr2(13983) ; rat ... Esr2(25149)

설명

일반 설명

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.

특이성

Estrogen receptor β, long form, does not detect estrogen receptor alpha.

Predicted to cross-react with mouse and rat based on sequence homology.

면역원

Epitope: a.a.55-74(H) or 63-82 (R, M)

KLH-conjugated, synthetic peptide corresponding to amino acids 63-82 of rat and mouse estrogen receptor β and amino acids 55-74 of human estrogen receptor β.

애플리케이션

Detect Estrogen Receptor β using this Anti-Estrogen Receptor β Antibody, clone 68-4 validated for use in WB & IP.

Immunoprecipitation:
10 μL of a previous lot immunoprecipitated estrogen receptor β from 500 μg of MCF7 RIPA lysate.

품질

Evaluated by western blot on RIPA lysates from MCF-7 cells.

Western Blot Analysis:
A 1:500 to 1:2,000 dilution of this antibody detected estrogen receptor β in RIPA lysates from MCF-7 cells.

표적 설명

59kDa

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable