추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
5HH2-2A8, monoclonal
종 반응성
mouse, human, rat
기술
immunocytochemistry: suitable
western blot: suitable
동형
IgG1κ
GenBank 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... H2BC21(8349)
mouse ... H2Bc21(319190)
일반 설명
Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
특이성
Antibody recognizes Histone H2B near the C-terminus.
면역원
Epitope: C-terminus
Ovalbumin-conjugated synthetic linear peptide corresponding to the C-terminus of Histone H2B.
애플리케이션
Anti-Histone H2B Antibody, clone 5HH2-2A8 is a Mouse Monoclonal Antibody for detection of Histone H2B also known as Histone H2B, histone 2 H2be & has been validated in WB, ICC.
Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
품질
Evaluated by western blot in HepG2 cell lysate.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.
표적 설명
~15 kDa
결합
Replaces: MABE15
물리적 형태
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate
HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate
법적 정보
GenBank is a registered trademark of United States Department of Health and Human Services
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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문서
Loading controls in western blotting application.
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