05-1923

Anti-phospho-Threonine Antibody, clone 20H6.1

clone 20H6.1, from mouse

• 동의어(들): Threonine
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 생산 유형: primary antibodies
• 클론: 20H6.1, monoclonal
• 종 반응성: mouse, human
• 종 반응성(상동성에 의해 예측): vertebrates (based on 100% sequence homology), all
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG2bκ
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pThr)

설명

일반 설명

Protein phosphorylation is normally associated with protein modification in signaling pathways and may be useful in pathophysiology and treatment of major neuropsychiatric disorders. Antibodies against phospho-threonine have been used to establish stability of the phosphorylated forms of proteins that may be involved in many different types of signaling. Phospho-threonine antibody is used to measure phosphorylation levels of PSII reaction center proteins D1, D3 and chlorophyll a binding protein CP43.

면역원

KLH-conjugated linear peptide corresponding to Threonine.

애플리케이션

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Threonine from 100 µg of A431 cells stimulated with calyculin/okadaic acid.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Threonine in A431, NIH/3T3, and HeLa cells.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

This phospho-Threonine antibody is validated for use in WB & ICC for the detection of the phospho-Threonine protein.

품질

Evaluated by Western Blot in calyculin/okadaic acid treated and untreated A431 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Threonine on 10 µg of calyculin/okadaic acid treated and untreated A431 cell lysates.

표적 설명

Various observed

물리적 형태

Format: Purified

Protein G

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Calyculin/okadaic acid treated and untreated A431 cell lysates.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable