05-649

Anti-Chk2 Antibody, clone 7

clone 7, Upstate^®, from mouse

• 동의어(들): CHK2 (checkpoint, S.pombe) homolog, CHK2 checkpoint homolog (S. pombe), checkpoint-like protein CHK2, protein kinase CHK2, serine/threonine-protein kinase CHK2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 7, monoclonal
• 종 반응성: mouse, human
• 제조업체/상표: Upstate^®
• 기술: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1
• NCBI 수납 번호: NP_001005735
• UniProt 수납 번호: O96017
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CHEK2(11200)

설명

일반 설명

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.

특이성

Chk2

Others not tested.

Predicted to cross-react with rat based on sequence homology.

면역원

Mixture of two GST fusion proteins corresponding to residues 1-223 and 217-543 of human Chk2

애플리케이션

Anti-Chk2 Antibody, clone 7 is a mouse monoclonal antibody for detection of Chk2 also known as CHK2 checkpoint homolog (S. pombe), serine/threonine-protein kinase CHK2 & has been validated in WB, IP, ICC.

Immunoprecipitation: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate Chk2 from HeLa cells.

Immunocytochemistry: A previous lot of this antibody has been reported by an independent laboratory to detect Chk2 in HeLa cells.

Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Chk2 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

품질

Routinely evaluated by western blot on RIPA lysates from Jurkat cells, HeLa, 3T3/A31, or A431 cells.

Western Blot Analysis: 0.1-1 μg/mL of this lot detected Chk2 in RIPA lysates from Jurkat cells. A previous lot detected Chk2 in lysates from HeLa, 3T3/A31, and A431 cells.

표적 설명

67 kDa

결합

Replaces: MABE491

물리적 형태

Format: Purified

Protein G Chromatography

Protein G purified mouse IgG1 buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.

저장 및 안정성

Stable for 1 year at from date of receipt.

분석 메모

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1