05-858

Anti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonal

clone 62-141-13, Upstate^®, from rabbit

• 동의어(들): H4, Histone H4, H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 62-141-13, monoclonal
• 종 반응성: chicken, Saccharomyces cerevisiae, vertebrates, yeast, human
• 제조업체/상표: Upstate^®
• 기술: ChIP: suitable (ChIP-seq); multiplexing: suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_003529
• UniProt 수납 번호: P62805
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: Saccharomyces cerevisiae ... Hhf1(852294) ; human ... H4C1(8359)

설명

일반 설명

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

This antibody recognizes histone H4 independant of any post-translational modifications.

특이성

Broad species cross-reactivity expected based on sequence homology.

Recognizes Histone H4, Mr 10 kDa.

면역원

Epitope: a.a. 25-28

KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28.

애플리케이션

Anti-Histone H4 Antibody, pan, clone 62-141-13 is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex, ChIP-seq.

Epitope Mapping (using Luminex^®):
1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).

Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

품질

Routinely evaluated by western blot.

Western Blot Analysis:
A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)

표적 설명

10 kDa

물리적 형태

Format: Purified

Protein A purified

Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated).

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

Luminex is a registered trademark of Luminex Corp

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable